yfjR [2019-06-25 11:01:12]

similar to 3-hydroxyisobutyrate dehydrogenase

locus: BSU07990

pI: 4.78

mw: 27.72 kDa

protein length: 286 aa

gene length: 861 bp

function: unknown

product: 3-hydroxyisobutyrate dehydrogenase

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 870,388 871,248

The protein

Protein family

• 3-hydroxyisobutyrate dehydrogenase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|1010B1028A9A5F234C33474C7BC23C9BD2ABF3C4|YkwC]:

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

Structure

• [PDB|4GBJ] (from ''Dyadobacter Fermentans'', 41% identity)

Expression and Regulation

Operons

• genes: [gene|9F8A63C585227D59C8A5CD4356E3F81EB5897131|yfjR]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|706983E6942E883D3A9D45693E7B4015AEABE60B|YclJ]: activation, [Pubmed|20512483], in [regulon|706983E6942E883D3A9D45693E7B4015AEABE60B|YclJ regulon]

Biological materials

Mutant

• MGNA-C350 (yfjR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2348 NBRP B. subtilis, Japan]
• BKE07990 ([gene|9F8A63C585227D59C8A5CD4356E3F81EB5897131|yfjR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCACTCCCTGAA, downstream forward: _UP4_TAAAGAAAAGCCTCTCCGTT
• BKK07990 ([gene|9F8A63C585227D59C8A5CD4356E3F81EB5897131|yfjR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCACTCCCTGAA, downstream forward: _UP4_TAAAGAAAAGCCTCTCCGTT

References

• 16493705,20512483